Studies on Optimization of Culture Conditions and Medium Components for the Production of Mycelial Biomass of Auricularia delicata under Submerged Fermentation
Muharagi Samwel Jacob
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, China.
Li Xiao *
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, China. and Key Laboratory of Edible Fungi Resources and Utilization (North), Ministry of Agriculture and Rural Areas, Changchun, Jilin 130118, China. and Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Changchun, Jilin 130118, China.
Mabagala Frank Stephano
College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China.
Xu Anran
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, China.
*Author to whom correspondence should be addressed.
Abstract
Aims: To optimize the culture conditions and medium components for the production of mycelial biomass of A. delicata under submerged fermentation.
Place and Duration of Study: China–Zambia Agricultural demonstration center and Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, China between July 2019 and June 2020.
Methodology: In this study, a single factor at a time method was employed in the optimization of submerged culture conditions and medium components for the production of mycelial biomass of A. delicata (strain YD 99). Each factor was screened independently while other factors were kept constant.
Results: The findings of this study demonstrate that the optimal culture conditions obtained were as follows: carbon source (Glucose) 20 gl-1, pH 6.0, nitrogen source (Yeast extract) 2 gl-1, mineral elements (K2HPO4+MgSO4.7H20) 2gl-1, and incubation temperature 25°C. The application of these optimal culture conditions produced a maximum concentration of 7.34gl-1 mycelial biomass of A. delicata.
Conclusion: Consequently, our results indicated that the optimization of culture conditions and medium components is of significant importance for the cultivation of A. delicata.
Keywords: Auricularia delicate, mycelial biomass, submerged fermentation, optimal culture condition.