Preparation and Vibrio sp Antibacterial Activity of Silver Nanoparticles Mediated by Chromolaena odorata Leaf Extract using Different Temperatures
Emsal Yanuar
Department of Metallurgy, Sumbawa University of Technology, Sumbawa, Indonesia.
Khotibul Umam
Department of Biotechnology, Sumbawa University of Technology, Sumbawa, Indonesia.
Wirya Sarwana
Department of Metallurgy, Sumbawa University of Technology, Sumbawa, Indonesia.
Ihsanul Huda
Department of Metallurgy, Sumbawa University of Technology, Sumbawa, Indonesia.
Dani Wijaya
Department of Biotechnology, Sumbawa University of Technology, Sumbawa, Indonesia.
Roto Roto *
Department of Chemistry, Gadjah Mada University, Sekip Utara, Yogyakarta, Indonesia.
Mudasir Mudasir *
Department of Chemistry, Gadjah Mada University, Sekip Utara, Yogyakarta, Indonesia.
*Author to whom correspondence should be addressed.
Abstract
This research aims to synthesize silver nanoparticles (AgNPs) using Kirinyuh (Chromolaena odorata) leaf extract with different temperature and evaluate its activity in Vibrio sp.
The synthesis was carried out by combining between silver nitrate solution and Kirinyuh leaf extract at room temperature (AgNPs 200) and hydrothermal process at 60oC (AgNPs 200*). Based on the results of LC-MS analysis, most of the chemical content of an extract was a flavonoid group compound. The color of the solution changed from colorless to red-brown, and a new peak around 435 nm showed that silver nanoparticles have been successfully obtained. The hydrothermal process exhibits sharp peaks rather than without hydrothermal treatment. The silver nanoparticles have a definite crystalline structure. The FTIR spectroscopy analysis indicated the organic compound was responsible for capping and stabilizing agents. In addition,TEM analysis showed that AgNPs 200 and AgNPs 200* exhibited average particle size of 32.89 nm and 27.82 nm, respectively. The presence of AgNPs can enhance the zone of growth inhibition of Vibrio sp approximately 300% compared to Kirinyuh leaf extract. Silver nanoparticles is estimated to possess the potential to be further evolved and utilized to inhibit the growth of Vibrio sp bacteria in shrimp agriculture.
Keywords: Green synthesis, Chromolaena odorata, silver nanoparticles, vibrio sp, temperatures