Analysis of the Influence of Zn Excess in the Pineal Gland by Total Reflection X-ray Fluorescence
Correa-Gillieron, Elenice Maria *
Federal University of Rio de Janeiro, Institute of Medical Biochemistry Leopoldo de Meis (IBqM), Center of Health Science, Rio de Janeiro, RJ, Brazil and Teacher in the Professional Master´s Degree in Education Management and Diffusion in Biosciences (MPEGeD), Institute of Medical Biochemistry Leopoldo de Meis, Center of Health Science, University City, Av. Carlos Chagas, 373, Zip Code 21-941-590, Ilha do Fundão, Rio de Janeiro, RJ, Brazil.
Barbosa, Renata Faria
Multidisciplinary Department, Federal University of Sao Paulo, Osasco, SP, Brazil and Multidisciplinary Department, Paulista School of Politics, Economics and Business, R. Oleska Winogradow 100, Jardim das Flores, Osasco, zip code 06110295, São Paulo, Brazil.
Ferezin-Pinto, Caroline
Oswaldo Cruz Foundation-Fiocruz, Bio-Manguinhos, Rio de Janeiro, RJ, Brazil and Department of Marked Relations, Institute of Technology in Immunobiologycals, Commercial Division, Bio-Manguinhos, Fundação Oswaldo Cruz – Fiocruz Av, 4365, Manguinhos, Rio de Janeiro, RJ, zip code 21040-900, Brazil.
Martinez, Ana Maria Blanco
Department of Pathology, Faculty of Medicine, Federal University of Rio de Janeiro, Neurodegeneration and Repair Laboratory, University Hospital Clementino Fraga Filho, Center of Health Science, Brazil and Department of Pathology, Faculty of Medicine, University Hospital Clementino Fraga Filho, University City, R. Prof. Rodolpho Rocco 255, zip code 21941-913, Ilha do Fundão, Rio de Janeiro, RJ, Brazil.
*Author to whom correspondence should be addressed.
Abstract
The TXRF technique (total reflection X-ray fluorescence) was employed to analyze the concentration of zinc (Zn) and other metals in the pineal gland of rats submitted to orally administered excess dose of Zn sulfate. The histochemical localization of Zn was also performed. TXRF results showed a 42.9% increase in Zn concentration, and alterations on the homeostasis of other essential elements in rats. It was concluded that TXRF is a suitable technique for measuring, for the first time in this work, the concentration of Zn accumulated in the pineal gland after administration of an excess dose of Zn, and this result may be either directly or indirectly related to alteration in the homeostasis of other chemical elements, such as S, Cl, K, Ca, Ti, Cr, Mn and Fe.
Keywords: Pineal, rat, Zinc, TXRF, hyperzincemia