Evaluation of the Ex vivo and In vitro Antidiabetic and Antioxidant Activities of the Hydroalcoholic Extract of Psidium guajava L. Leaves (Myrtaceae)
Afangbom Kossi *
Département de Physiologie Animale, Faculté des Sciences, Université de Lomé, 01BP 1515-Lomé 01, Togo and Département de Biochimie, Faculté des Sciences, Université de Lomé, 01BP 1515-Lomé 01, Togo.
Motto Aku Enam
Département de Biochimie, Faculté des Sciences, Université de Lomé, 01BP 1515-Lomé 01, Togo.
Melila Mamatchi
Département de Physiologie Animale, Faculté des Sciences, Université de Lomé, 01BP 1515-Lomé 01, Togo.
Atchou Kokou
Département de Biochimie, Faculté des Sciences, Université de Lomé, 01BP 1515-Lomé 01, Togo.
Lawson-EVI Povi
Département de Biochimie, Faculté des Sciences, Université de Lomé, 01BP 1515-Lomé 01, Togo.
*Author to whom correspondence should be addressed.
Abstract
Objective: Antidiabetic activity was assessed by the skeletal muscle glucose uptake test and antioxidant potential by the total antioxidant capacity, reducing power and DPPH test.
Materials and Methods: Same-sex Sprague Dawley rats weighing 150±20 g and approximately 3 months old and different concentrations of the extract obtained from 500 g of P. guajava leaves powder were used for the following methods: reduction, free radical scavenging, glucose uptake by skeletal muscle.
Results: In terms of antioxidant potential, the extract showed significant activity in a dose-dependent manner. In addition, the extract potentiated glucose uptake in the presence and absence of insulin. Compared with metformin and glibenclamide, the extract increased glucose uptake to a greater extent.
Conclusion: This study shows that the hydroethanolic extract of guava leaves is one of the best recipes for helping to delay the complications associated with diabetes.
Keywords: Diabetes, Psidium guajava, antioxidant activity, uptake of glucose