DPPH and ABTS Radical Scavenging Assay and Hemolytic Potential of Methanol Leaf Extract of Erythrina crista-galli L.
Keerthana H. Deshpande
Department of Botany, B.M.S College for Women (Autonomous), Basavanagudi, Bengaluru–560 004, India.
Syed Muskan Imran
Department of Botany, B.M.S College for Women (Autonomous), Basavanagudi, Bengaluru–560 004, India.
B. S. Ramesh *
Department of Botany, Universal school of administration, Ramohalli, Bengaluru - 560074, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Erythrina crista-galli is a medicinal plant containing bioactive constituents of potential biological importance. Evaluation of its radical-scavenging activity and interaction with red blood cells is important for characterising the antioxidant activity and haemolytic potential of its leaf extract.
Aim: The present study aims to investigate the antioxidant activity and haemolytic potential of the methanol leaf extract of Erythrina crista-galli.
Method: Anti-radical activity was assessed using the DPPH and ABTS radical scavenging assays, and the haemolytic activity of the methanol leaf extract was evaluated.
Results: The methanol leaf extract of E. crista-galli demonstrated DPPH scavenging activity; at extract concentrations of 6.25, 12.5, 25, 50, and 75 µg/mL, the recorded inhibition values were 3.125%, 15.625%, 25.0%, 31.25%, and 37.5%, showing a gradual enhancement in antioxidant potential with increasing concentration. The percentage inhibition of ABTS radicals increased progressively with increasing extract concentration, indicating a concentration-dependent antioxidant effect. Quercetin was used as the standard. The plant extract exhibited a concentration-dependent increase in haemolytic activity.
Conclusion: The present findings confirm the existence of mild and remarkable antioxidant activity and significant haemolytic potential in the leaf extract of E. crista-galli, and these data could pave the way for further research.
Keywords: Erythrina crista-galli, methanol leaf extract, DPPH assay, ABTS assay, radical-scavenging activity, antioxidant activity